Mouse anti‑Chicken Actin IgG1,k Monoclonal RW-C63539-100

Antibody: Actin Mouse anti-Chicken Monoclonal (aa50-70) Antibody, Application: IHC, IHC-Fr, IF, WB, ELISA, Reactivity: Chicken, Format: Unconjugated, Unmodified, Descriptio: Actin antibody RW-C63539 is an unconjugated mouse monoclonal antibody to chicken Actin (aa50-70). Validated for ELISA, IF, IHC and WB., Targe: Chicken Actin, Hos: Mouse, Reactivit: Chicken (tested or 100% immunogen sequence identity), Clonalit: IgG1,k Monoclonal, Conjugation: Unconjugated, Purificatio: Ascites, Modification: Unmodified, Immunoge: Purified chicken gizzard actin., Epitop: aa50-70, Specificit: Appears to be specific for the N-terminal two-thirds of the actin molecule (possibly near residues 50-70 in primary sequence). Reacts with every actin form tested. Species cross-reactivity: Reacts with all animal species and cell types with an actin form, including plant actin., Application: IHC
IHC - Frozen
Immunofluorescence (1:100)
Western blot (1:100 - 1:1000)
ELISA, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Suitable for use in Indirect Immunofluorescence, Western Blot, Iodination and Solid Phase Binding. Indirect immunofluorescence: 1:100; Tissue culture cells-fix with formaldehyde, treat with methanol or acetone. Glycerinated myofibrils-fix fibers with formaldehyde, treat with cold methanol. Stains I-bands intensely and stress fibers in human fibroblasts. Cryostat sections (6um)-quick frozen in isopentane, slides treated with gelatin and formaldehyde. Western Blots: 1:100-1:1000; On muscle homogenates subject to SDS-PAGE, reacts relatively uniformly with a 43kD protein present in skeletal, cardiac, gizzard and aorta tissues. Appears to react with all isoforms of actin found in these preparations and shows a strong reaction with the alpha actin found in skeletal, cardiac and arterial muscle. Solid Phase Binding Assay: 1:800-1:1000; In an ELISA, is strongly reactive with the cytoplasmic actin and shows a significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows a significant binding to both Dictyostelium discoideum and Physarum polycephalum., Presentatio: 0.01% Sodium Azide, Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.