Mouse anti‑Chicken Actin IgG2b,k Monoclonal RW-C63540-100

Antibody: Actin Mouse anti-Chicken Monoclonal (N-Terminus) Antibody, Application: IHC, IHC-P, ICC, WB, ELISA, Reactivity: Chicken, Human, Mouse, Rat, Guinea pig, Rabbit, Sheep, Format: Unconjugated, Unmodified, Other formats: APC Biotin FITC HRP PE, Descriptio: Actin antibody RW-C63540 is an unconjugated mouse monoclonal antibody to Actin (N-Terminus) from chicken. It is reactive with human, mouse, rat and other species. Validated for ELISA, ICC, IHC and WB., Targe: Chicken Actin, Hos: Mouse, Reactivit: Chicken, Human, Mouse, Rat, Guinea pig, Rabbit, Sheep (tested or 100% immunogen sequence identity), Clonalit: IgG2b,k Monoclonal, Conjugation: Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE., Purificatio: Protein A purified, Modification: Unmodified, Immunoge: Purified chicken gizzard actin. Epitope: Appears to be located in the N-Terminus two thirds of the actin molecule, possibly near aa50-70., Epitop: N-Terminus, Specificit: Recognizes all six isoforms of vertebrate actin, globular (G) and filamentous (F) forms. This clone is a pan-actin antibody that binds to an epitope in a highly conserved region of actin. Labels myotubes and stains myoblasts and fibroblasts. Does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. Species cross-reactivity: All species., Application: IHC
IHC - Paraffin
ICC (1 - 20 µg/ml)
Western blot
ELISA, Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more: None, Usag: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and ELISA. Immunohistochemistry: 10 ug/ml (paraffin embedded, 4% formaldehyde, 3% glutaraldehyde, sodium cacodylate treated sections). Visualized using a donkey anti-mouse IgG (Cy3). Positive staining pattern reflects both membrane and cytoplasmic staining. ELISA: Shows a strong reactivity with cytoplasmic actin and significant binding to gizzard, skeletal, arterial and cardiac actins. Also shows significant binding to both Dictyostelium discoideum and Physarum polycephalum in ELISA., Presentatio: 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, 0.05% Sodium Azide, Restriction: For research use only. Intended for use by laboratory professionals., Guarante: This antibody carries the RW 100% Guarantee.